Data/hora: 13/06/2024 23:41:30

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum	
Autores: 	Pérez,Edmundo A.; Fernández,Francisco J.; Fierro,Francisco; Mejía,Armando; Marcos,Ana T.; Martín,Juan F.; Barrios-González,Javier.	
Data: 	2014-09-01	
Ano: 	2014	
Palavras-chave: 	Glucose regulation; Glucokinase; Penicillin; Penicillium chrysogenum.	
Resumo: 	The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose regulation of penicillin biosynthesis. Our results show that Penicillium chrysogenum AS-P-78 and dogR5 strains lack hexokinase, and suggest that an enzyme with glucokinase activity is involved in glucose regulation of penicillin biosynthesis and β-galactosidase, thus signaling glucose in both primary and secondary metabolism; however, catalytic and signaling activities seem to be independent.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300017	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822014000300017	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.45 n.3 2014	
Fonte: 	info:eu-repo/semantics/openAccess