Data/hora: 01/06/2024 10:31:26

Provedor de dados: | 58
País: | Brazil
Título: | Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
Autores: | Schneid,Andréa dos Santos; Rodrigues,Kelly Lameiro; Chemello,Davi; Tondo,Eduardo Cesar; Ayub,Marco Antônio Zacchia; Aleixo,José Antonio Guimarães.
Data: | 2006-09-01
Ano: | 2006
Palavras-chave: | Salmonella; Monoclonal antibody; Policlonal antibody; ELISA.
Resumo: | In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822006000300027
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.37 n.3 2006
Fonte: | info:eu-repo/semantics/openAccess