Data/hora: 02/06/2024 11:30:42

Provedor de dados: | 58
País: | Brazil
Título: | Polyphasic characterization of bacteria obtained from upland rice cultivated in Cerrado soil
Autores: | Braga,Lívia Fabiana; Oliveira,Fênix Araújo de; Couto,Eva Aparecida Prado do; Santos,Karina Freire d'Eça Nogueira; Ferreira,Enderson Petrônio de Brito; Martin-Didonet,Claudia Cristina Garcia.
Data: | 2018-03-01
Ano: | 2018
Palavras-chave: | Oryza sativa; PGPR; Associative bacteria; Bacterial diversity.
Resumo: | ABSTRACT This work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, catalase and, nitrogenase. All isolates were able to metabolize sucrose and malic acid in contrast with mannitol, which was metabolized only by one isolate. For the other carbon sources, we observed a great variability in its use by the isolates. Most isolates showed antibiosis against Rhizoctonia solani (75%) and Sclerotinia sclerotiorum (55%) and, 50% of them showed antibiosis against both pathogens. Six isolates showed simultaneous ability of antibiosis, inorganic phosphate solubilization and protease activity. Based on phylogenetic analysis of the 16S rRNA gene all the isolates belong to Bacillus genus. Under greenhouse conditions, two isolates (S4 and S22) improved to about 24%, 25%, 30% and 31% the Total N, leaf area, shoot dry weight and root dry weight, respectively, of rice plants, indicating that they should be tested for this ability under field conditions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100020
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2017.04.004
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.49 n.1 2018
Fonte: | info:eu-repo/semantics/openAccess