Data/hora: 03/06/2024 08:03:36

Provedor de dados: | 59
País: | Brazil
Título: | Use of remazol blue dyed avicel for the determination of cellulolytic activity in basidiomycetes
Autores: | Correia,Marcos José; Pereira Junior,José Antônio de Sousa; Santos,Jefferson Cunha dos; Cavalcanti,Maria Auxiliadora de Queiroz.
Data: | 1998-10-01
Ano: | 1998
Palavras-chave: | Avicel; Cellulase; Remazol Blue; Lentinus edodes; Pleurotus ostreatus.
Resumo: | A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes is discussed. Refinements were introduced in a simple method for quantitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by the enzymatic hydrolysis. Modifications in Avicel-dye preparation were enhanced and a spectrophotometer for direct OD measurement in agar test tubes used. The use of a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maximum wavelenght for RBBR (595 nm).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400010
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141998000400010
Formato: | text/html
Fonte: | Revista de Microbiologia  v.29 n.4 1998
Fonte: | info:eu-repo/semantics/openAccess