Data/hora: 01/06/2024 02:18:21

Provedor de dados: | 59
País: | Brazil
Título: | Bacteria isolated from a sugarcane agroecosystem: their potential production of polyhydroxyalcanoates and resistance to antibiotics
Autores: | Lima,Teresa Cristina S. de; Grisi,Breno M.; Bonato,M. Christina M.
Data: | 1999-07-01
Ano: | 1999
Palavras-chave: | Soil bacteria poly-&lt; FONT FACE=Symbol&gt; B&lt; /font&gt; -hydroxyalkanoate PHA resistance to antibiotics sugarcane agroecosystem vinasse.
Resumo: | In this investigation, a sugarcane agroecosystem at a coastal tableland, in the northeast of Brazil, was screened to obtain bacteria strains able to synthesize poly-&lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;-hydroxyalkanoates (PHA), using sucrose as the main carbon source. The potential to synthesize PHA was tested qualitatively by Sudan Black staining of colonies growing in different carbon sources: sucrose, glucose, fructose, propionate and cellulose. In a typical sugarcane crop management system, the plantation is burned before harvesting and vinasse, a byproduct of alcohol production, is used in a fertirrigation system causing, probably, selective pressures on the microbiota of natural environments. Eightytwo bacteria strains, belonging to 16 different genera and 35 different species, were isolated. The data showed that 11 strains (ca 13%), nine of which belonging to the genus Pseudomonas, presented a strong Sudan Black staining in several carbon sources tested and, simultaneously, showed multiple resistance to antibiotics. Resistance to antibiotics is an advantageous feature for the biotechnological production of PHAs. The total number of isolates with multiple resistance to antibiotics was 73, and 38% of them belong to the genus Pseudomonas. Among the isolates, ca 86% and 43% grew in the presence of 10-100 U/ml of penicillin and/or 100-300 mg/ml of virginiamycin, respectively. These antibiotics are utilized in the alcohol distillery we investigated. The results suggest that some agroecosystem environments could be considered as habitats where bacteria are submitted to nutritional unbalanced conditions, resulting in strains with potential ability to produce PHAs, and also, to an increase in the microbial diversity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300006
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141999000300006
Formato: | text/html
Fonte: | Revista de Microbiologia  v.30 n.3 1999
Fonte: | info:eu-repo/semantics/openAccess