Data/hora: 03/06/2024 11:05:49

Provedor de dados: | 59
País: | Brazil
Título: | Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
Autores: | Cassiolato,Ana Maria R.; Melo,Itamar Soares de.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Filtration enrichment technique; Auxotrophic mutants; Trichoderma harzianum; Benomyl.
Resumo: | The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500µg/L of benomyl.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100008
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141999000100008
Formato: | text/html
Fonte: | Revista de Microbiologia  v.30 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess