Data/hora: 16/06/2024 05:10:13

Provedor de dados: | 59
País: | Brazil
Título: | Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius
Autores: | Silva,Claudio Henrique Cerri e; Puls,Jurgen; Sousa,Marcelo Valle de; Ferreira Filho,Edivaldo Ximenes.
Data: | 1999-04-01
Ano: | 1999
Palavras-chave: | Xylan; Xylanase; Aspergillus fumigatus.
Resumo: | A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 °C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligomers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase activity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200005
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141999000200005
Formato: | text/html
Fonte: | Revista de Microbiologia  v.30 n.2 1999
Fonte: | info:eu-repo/semantics/openAccess