Data/hora: 01/06/2024 02:46:20

Provedor de dados: | 59
País: | Brazil
Título: | Plasmids in Mycoplasma species isolated from goats and sheep and their preliminary typing
Autores: | Nascimento,Elmiro R.; DaMassa,Al J.; Yamamoto,Richard; Nascimento,M. Graça F.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Mycoplasma; Plasmid; Goat; Endonuclease; Electrophoresis.
Resumo: | One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the linearized supercoiled extrachromosomal DNA, five plasmid types were recovered (p1II, p2III, p2V, p3I, and p4IV). The small size of these DNA elements probably exclude replicative forms of DNA virus, which are equal or larger than 8.0 Kbp.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100006
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141999000100006
Formato: | text/html
Fonte: | Revista de Microbiologia  v.30 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess