Data/hora: 05/06/2024 18:19:37

Provedor de dados: | 59
País: | Brazil
Título: | Characterization of fusion products from protoplasts of yeasts and their segregants by electrophoretic karyotyping and RAPD
Autores: | Martins,Cleide Viviane Buzanello; Horii,Jorge; Pizzirani-Kleiner,Aline Aparecida.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Protoplast fusion; Electrophoretic karyotyping; RAPD.
Resumo: | In order to characterize fusion products from yeast protoplasts and their segregants, with important features to the wine making industry, electrophoretic karyotyping and RAPD (Random Amplified Polymorphic DNA) were utilized. Electrophoretic karyotyping was performed by the CHEF ("contour-clamped homogeneous electric field electrophoresis") method, which allowed the detection of chromosomal band complementation in fusion products and the presence of patterns of both parental and intermediary strains in segregants. By utilizing two primers, an amplification pattern of DNA fragments was obtained. While fusion products (diploid) showed a pattern of complementary bands, segregants showed bands of either parental strains or even intermediary bands
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100014
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141999000100014
Formato: | text/html
Fonte: | Revista de Microbiologia  v.30 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess