Data/hora: 05/06/2024 17:58:09

Provedor de dados: 	60	
País: 	Brazil	
Título: 	A simple method for the quantification of diclofenac potassium in oral suspension by high-performance liquid chromatography with UV-detection	
Autores: 	Rubim,Alexandre Machado; Rubenick,Jaqueline Bandeira; Laporta,Luciane Varine; Rolim,Clarice Madalena Bueno.	
Data: 	2013-09-01	
Ano: 	2013	
Palavras-chave: 	High performance liquid chromatography; Diclofenac potassium; Oral suspension.	
Resumo: 	A rapid, simple and low cost method was developed to determine diclofenac potassium (DP) in oral suspension, using a reverse-phase column (C8, 150 mm x 4.6 mm, 5 µm), mobile phase containing methanol/buffer phosphate (70:30 v/v, pH 2.5), at a flow rate of 1.0 mL/min, isocratic method, and ultraviolet detection at 275 nm. A linear response (r = 1.0000) was observed in the range of 10.0-50.0 µg/mL. Validation parameters such as linearity, specificity, precision, accuracy and robustness were evaluated. The method presented precision (repeatability: relative standard deviation = 1.21% and intermediate precision: between-analyst = 0.85%). The specificity of the assay was evaluated by exposure of diclofenac potassium under conditions of stress such as hydrolysis, photolysis, oxidation and high temperature. The method presented accuracy values between 98.28% and 101.95%. The results demonstrate the validity of the proposed method that allows determination of diclofenac potassium in oral suspension and may be used as an alternative method for routine analysis of this product in quality control.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300021	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/S1984-82502013000300021	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.49 n.3 2013	
Fonte: 	info:eu-repo/semantics/openAccess