Data/hora: 07/06/2024 02:53:42

Provedor de dados: | 60
País: | Brazil
Título: | Protein PEGylation for the design of biobetters: from reaction to purification processes
Autores: | Santos,João Henrique Picado Madalena; Torres-Obreque,Karin Mariana; Meneguetti,Giovanna Pastore; Amaro,Beatriz Panichi; Rangel-Yagui,Carlota Oliveira.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | PEGylation; Biobetters; Biological drugs; Polyethylene glycol; Protein purification; Site-specific PEGylation.
Resumo: | The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and pharmacodynamics characteristics of the protein drug. Nonetheless, correct information in terms of the PEGylation process from reaction to downstream processing is of paramount importance for the industrial application and processing scale-up. In this review we present and discuss the main steps in protein PEGylation, namely: PEGylation reaction, separation of the products and final characterization of structure and activity of the resulting species. These steps are not trivial tasks, reason why bioprocessing operations based on PEGylated proteins relies on the use of analytical tools according to the specific pharmaceutical conjugate that is being developed. Therefore, the appropriate selection of the technical and analytical methods may ensure success in implementing a feasible industrial process.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700408
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/s2175-97902018000001009
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.54 n.spe 2018
Fonte: | info:eu-repo/semantics/openAccess