Data/hora: 15/06/2024 02:40:12

Provedor de dados: | 60
País: | Brazil
Título: | Development of agar diffusion method for dosage of gramicidin
Autores: | Solano,Ana Gabriela Reis; Pereira,Larissa de Melo Campos Sousa; Leonel,Míriam de Fátima Vianna; Nunan,Elzíria de Aguiar.
Data: | 2011-09-01
Ano: | 2011
Palavras-chave: | Gramicidin/microbiological assay; Microbiological assay/method validation; Agar diffusion; Cylinder-plate.
Resumo: | Gramicidin, an antimicrobial peptide active against Gram positive bacteria, is commonly used in pharmaceutical preparations for topical use. Considering that only the turbidimetric method has been described in the literature, the present study sought to develop and validate an agar diffusion method for the dosage of gramicidin. The method was developed and validated using the Kocuria rhizophila ATCC 9341 as a test microorganism. Two designs were used: a 3x3 parallel-line model, and a 5x1 standard curve. The validation demonstrated that the method follows the linear model (r²= 0.994), presenting a significant regression between the zone diameter of growth inhibition and the logarithm of the concentration within the range of 5 to 25.3 µg/mL. The results obtained for both designs were precise, having a relative standard deviation (R.S.D.) for intra-day precision of 0.81 for the 3x3 assay and 1.90 for the 5x1 assay. For the inter-day precision, the R.S.D. was 1.35 for the 3x3 and 2.64 for the 5x1. The accuracy was verified and results confirmed to be accurate, having a tolerance interval of 95%, which lay within permitted limits and appropriate trueness. In addition, the method was considered selective, with limit of detection and upper and lower limits of quantification of 2.00, 5.00 and 25.3 µg/mL, respectively. No difference in precision between the designs used in the agar diffusion method was evident (p&gt;0.05). The method proved to be appropriate for the microbiological dosage of the raw material gramicidin.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300014
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502011000300014
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.47 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess