Data/hora: 02/06/2024 14:53:49

Provedor de dados: 	60	
País: 	Brazil	
Título: 	An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules	
Autores: 	Silva,Pedro Henrique Reis da; Enéas,Paula Cristina Rezende; Fernandes,Christian; Pianetti,Gerson Antônio.	
Data: 	2017-01-01	
Ano: 	2017	
Palavras-chave: 	Prednisone; Capsules; High performance liquid chromatography/validation; Linearity assessment.	
Resumo: 	ABSTRACT Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2&gt;0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD&lt;2.0%), robustness, and accuracy (average recovery of 100.05%). The chromatographic procedure was applied for assay and uniformity content determination of three different batches of prednisone capsules, showing to be suitable for their quality control.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200624	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s2175-97902017000216064	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.53 n.2 2017	
Fonte: 	info:eu-repo/semantics/openAccess