Data/hora: 05/06/2024 04:01:46

Provedor de dados: | 60
País: | Brazil
Título: | Fluorometric quantification of protoporphyrin IX in biological skin samples from in vitro penetration/permeation studies
Autores: | Rossetti,Fábia Cristina; Depieri,Lívia Vieira; Tedesco,Antônio Cláudio; Bentley,Maria Vitória Lopes Badra.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Fluorometric quantification; Protoporphyrin IX/determination; Photodynamic therapy; Porcine skin; Cutaneous penetration/in vitro study; Cutaneous permeation/in vitro study.
Resumo: | A fluorometric analytical method was developed for quantification of protoporphyrin IX (PpIX) in skin samples and receptor phase solution after in vitro cutaneous penetration/permeation studies. Analytical conditions used were: excitation and emission wavelengths: 400 nm and 632 nm; bandwidth: 0.5 nm; excitation and emission slits: 10/10. PpIX was recovered from two different layers of skin, the stratum corneum (SC) and the epidermis plus dermis ([E+D]), by vortex homogenization, probe and bath sonication, using DMSO as an extraction solvent. The detection and quantification limits were 0.002 and 0.005 μg/mL, respectively. The assay was linear from 0.005 - 0.5 μg/mL. The within-day and between-day assay precision and accuracy in DMSO and receptor phase solution were each studied at the two concentration levels 0.04 and 0.2 μg/mL, and 0.01 and 0.08 μg/mL, respectively. The coefficients of variation and deviation from the theoretical values were lower than 5%. The skin recovery of PpIX from SC and [E+D] layers using two different concentrations (0.5 and 1.0 μg/mL) were all above 90.0%. The method described has potential application to in vitro penetration/permeation studies of PpIX using porcine skin as a biological membrane model.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400017
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502010000400017
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.46 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess