Data/hora: 01/06/2024 02:28:35

Provedor de dados: | 60
País: | Brazil
Título: | A study on enhanced intestinal permeability of clarithromycin nanoparticles
Autores: | Zakeri-Milani,Parvin; Islambulchilar,Ziba; Majidpour,Fatemeh; Jannatabadi,Ensieh; Lotfipour,Farzaneh; Valizadeh,Hadi.
Data: | 2014-03-01
Ano: | 2014
Palavras-chave: | Clarithromycin/nanoparticles/ permeability; Single-Pass Intestinal Perfusion; Intestinal permeability.
Resumo: | The main objective of the present study was to determine the permeability of clarithromycin (CLA)-PLGA nanoparticles using single-pass intestinal perfusion technique in rats. Clarithromycin nanoparticles were prepared by nano-precipitation according to the modified quasi emulsion solvent diffusion technique and evaluated for their physicochemical characteristics. Permeability coefficients (Peff) in anaesthetized rats were determined at 3 different concentrations. Drug solution or suspensions in PBS was perfused through a cannulated jejunal segment and samples were taken from outlet tubing at different time points up to 90 min. Microbiological assay of CLA and phenol red in the samples were analyzed using an agar well diffusion procedure and HPLC method respectively. The average particle size of prepared nanoparticles was 305 ± 134 nm. The mean Peff of CLA solution in concentrations of 150, 250 and 400 µg/mL was found to be 1.20 (±0.32) ×10-3, 9.62 (±0.46) ×10-4, and 1.36 (±0.95) ×10-3 cm/sec, respectively. The corresponding values for the same concentration of nanoparticles were found to be 2.74 (±0.73) ×10-3, 2.45 (±0.88) ×10-3, and 3.68 (±0.46) ×10-3 cm/s, respectively. The two-tailed Student’s t-test showed that the intestinal permeability of CLA nanoparticle suspensions in prepared concentrations were significantly increased in comparison with its solution.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100012
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502011000100012
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.50 n.1 2014
Fonte: | info:eu-repo/semantics/openAccess