Data/hora: 05/06/2024 14:07:51

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Quantitative analysis of phenobarbital in biological fluids: Analyte enrichment by an electrically-assisted microextraction technique	
Autores: 	Yaripour,Saeid; Ebrahimi,Shahriar; Mohammadi,Ali.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	Biological samples; Electromembrane extraction; HPLC-UV; Microextraction; Phenobarbital.	
Resumo: 	An electrically-assisted microextraction method called electromembrane extraction, followed by a simple high performance liquid chromatography and ultraviolet detection was developed and validated for determining phenobarbital in biological samples. The major parameters influencing the electromembrane extraction procedure including solvent composition, voltage, pH of acceptor and donor solutions, salt effect, and time of extraction were evaluated and optimized. The drug was extracted from the donor aqueous sample solution (pH 9) to the acceptor aqueous solution (pH 13). The donor and acceptor phases were separated by a hollow fiber dipped in 1-octanol as a supported liquid membrane. A voltage of 40 V during 20 minutes was applied as the driving force. The enrichment factor was obtained &gt;51 which enhanced the sensitivity of the instrument. Limit of detection and limit of quantitation were 7.5 and 25 ng/mL, respectively. The method was linear over the range of 25-1000 ng/mL for phenobarbital (R2 &gt;0.9998) with repeatability (%RSD) between 0.4% and 6.8% (n = 3). The proposed method was successfully applied to human plasma and urine samples with relative recovery of 70-80% and %RSD &lt; 6.8%.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100543	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s2175-97902019000417839	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.56 2020	
Fonte: 	info:eu-repo/semantics/openAccess