Data/hora: 01/06/2024 02:38:24

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Neuroprotective and MAOB inhibitory effects of a series of caffeine-8-thioglycolic acid amides	
Autores: 	Kasabova-Angelova,Alexandra; Kondeva-Burdina,Magdalena; Mitkov,Javor; Georgieva,Maya; Tzankova,Virginia; Zlatkov,Alexander.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	Neuroprotection; Caffeine; Synaptosomes; SH-SY5Y; HMAOB.	
Resumo: 	The effects of new derivatives of caffeine-8-thioglycolic acid (100 µM) on isolated rat brain synaptosomes, human neuroblastoma cell line SH-SY5Y and human recombinant MAOB enzyme (hMAOB) (1 µM) were evaluated. Most of the compounds, administered alone, didn’t show statistically significant neurotoxic effects on SH-SY5Y, when compared to the control (non-treated cells). Of all studied structures JTA-2Ox, JTA-11, JTA-12 and JTA-13 decreased cell viability. In combination with 6-hydroxydopamine (6-OHDA) (100 µM), only JTA-1 and JTA-2 revealed neuroprotective effects, stronger than those of caffeine. All compounds administered alone revealed, neurotoxic effects on synaptosomes, as compared to non-treated synaptosomes. JTA-1, JTA-2 and JTA-3 showed lowest neurotoxic effects and were investigated in a model of 6-OHDA-induced oxidative stress. In this model of neurotoxicity, only JTA-1 and JTA-2 showed statistically significant neuroprotective effect, by preserving the synaptosomal viability and the level of reduced glutathione. Inhibition of hMAOB, was revealed by JTA-1 and JTA-2. They inhibited the enzyme by 23% and 25% respectively, thus approaching the selegiline activity, which was 42%. The possible mechanisms of neuroprotection of JTA-1 and JTA-2 might be a result from the inhibition of hMAOB, which catalyze the production of neurotoxic p-quinone from 6-OHDA.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100529	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s2175-97902019000318255	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.56 2020	
Fonte: 	info:eu-repo/semantics/openAccess