Data/hora: 06/06/2024 02:27:23

Provedor de dados: | 60
País: | Brazil
Título: | Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules
Autores: | Queiroz,Kelly Marques; Silva,Maria-Luiza Martins; Prado,Nathália Duque; Lima,Paulo Marcelo Andrade; Diniz,Rosiane Dias Lopes; César,Isabela Costa; Pianetti,Gerson Antônio; Santos,Daniel Assis.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | Fluconazole/quantitative determination; Microbiological assay/agar diffusion; High performance liquid chromatography.
Resumo: | The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry laboratories.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400012
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502009000400012
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.45 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess