Data/hora: 01/06/2024 07:57:02

Provedor de dados: | 60
País: | Brazil
Título: | Macromolecular confinement of therapeutic protein in polymeric particles for controlled release: insulin as a case study
Autores: | Guerreiro,Luiz Henrique; Silva,Daniel da; Girard-Dias,Wendell; Mascarenhas,Camile Moreira; Miranda,Kildare; Sola-Penna,Mauro; Ricci Júnior,Eduardo; Lima,Luís Mauricio Trambaioli da Rocha e.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Human insulin/study; Microparticles; Poly-ε-caprolactone; Therapeutic proteins/study/action; Proteins/molecular entrapping.
Resumo: | ABSTRACT Sustained release systems for therapeutic proteins have been widely studied targeting to improve the action of these drugs. Molecular entrapping of proteins is particularly challenging due to their conformational instability. We have developed a micro-structured poly-epsilon-caprolactone (PCL) particle system loaded with human insulin using a simple double-emulsion w/o/w method followed by solvent evaporation method. This formulation is comprised by spheric-shaped microparticles with average size of 10 micrometers. In vitro release showed a biphasic behavior such as a rapid release with about 50% of drug delivered within 2 hours and a sustained phase for up to 48 h. The subcutaneous administration of microencapsulated insulin showed a biphasic effect on glycemia in streptozotocin-induced diabetic mice, compatible with short and intermediate-acting behaviors, with first transition peak at about 2 h and the second phase exerting effect for up to 48h after s.c. administration. This study reveals that a simplified double-emulsion system results in biocompatible human-insulin-loaded PCL microparticles that might be used for further development of optimized sustained release formulations of insulin to be used in the restoration of hormonal levels.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200622
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/s2175-97902017000216039
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.53 n.2 2017
Fonte: | info:eu-repo/semantics/openAccess