Data/hora: 11/06/2024 15:45:48

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Validation of spectrophotometric method to detect and quantify nitrite in ham pate	
Autores: 	Reis,Patrícia Barros; Ramos,Rodrigo Mauro; Souza,Leonardo Francisco de; Cançado,Silvana de Vasconcelos.	
Data: 	2009-03-01	
Ano: 	2009	
Palavras-chave: 	Validation; Nitrite; Spectrophotometric method; Ham pate.	
Resumo: 	The objective of this work was to validate the spectrophotometric method to detect and quantify nitrite using ham pate as a source. The validated analytical conditions were 540 nm wavelength and the samples reading between 40 to 70 minutes after addition of coloring agents. The tested performance criteria were: linearity, matrix effect, selectivity, detection and quantification limit, accuracy, precision and robustness. As the results have obtained linearity in the studied zone (0.125 to 3 g/mL) and matrix effect was not observed, there was not any interference from ascorbic acid, but interference occurred from sodium eritorbate, in concentrations above 2.5 mg/100g. The detection limit was defined as 12.5 mg of nitrite/kg for pate and the quantification limit as 25 mg of nitrite/kg of pate. The method presented repeatability and reproducibility. The accuracy was assessed through recovery values varying from 84 to 110%. Respecting robustness, it was observed that the variations applied to the extraction procedure depended on the concentration of nitrite in the pate sample. In conclusion, the methodology was validated under the tested conditions.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100006	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/S1984-82502009000100006	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.45 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess