Data/hora: 15/06/2024 03:04:55

Provedor de dados: | 60
País: | Brazil
Título: | Development and validation of a HPTLC method for analysis of Sunitinib malate
Autores: | Hajmalek,Monireh; Goudarzi,Masoumeh; Ghaffari,Solmaz; Attar,Hossein; Mazlaghan,Mehrnoosh Ghanbari.
Data: | 2016-12-01
Ano: | 2016
Palavras-chave: | Sunitinib malate/quantitative analysis; High Performance Thin Layer Chromatography/method/validation; Sunitinib malate/E-Z isomer.
Resumo: | ABSTRACT A simple high performance thin layer chromatography (HPTLC) has been developed and validated for determination of sunitinib malate and possible impurities. The samples were applied in forms of bands on an aluminum TLC plate pre-coated with silica gel and were separated using dichloromethane: methanol: toluene: ammonia solution as the mobile phase. Sunitinib malate was thoroughly separated from impurities including E-isomer, sunitinib N-oxide and impurity B with a retention factor (RF) of 0.35±0.02. Quantitative analysis of sunitinib was carried out using a mobile phase consisting of dichloromethane:methanol:ammonia solution, RF value was 0.53±0.02 for Z isomer. Detection was performed densitometrically in absorbance mode at 430 nm. This method was found to produce sharp, symmetrical, and well resolved peaks. Linear relationship with the coefficients of determination &gt; 0.99 was achieved over the concentration range of 27.34 to 437.5 ng/spot. This method provides robust, replicable and accurate results with acceptable sensitivity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400595
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/s1984-82502016000400003
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.52 n.4 2016
Fonte: | info:eu-repo/semantics/openAccess