Data/hora: 09/06/2024 15:57:05

Provedor de dados: | 60
País: | Brazil
Título: | Direct effect of  p,p'- DDT on mice liver
Autores: | Arroyo-Salgado,Bárbara; Olivero-Verbel,Jesús; Guerrero-Castilla,Angélica.
Data: | 2016-06-01
Ano: | 2016
Palavras-chave: | Pesticides; Persistent organic pollutant; Dichlorodiphenyltrichloroethane/exposure; Type 2 diabetes mellitus/exposure to dichlorodiphenyltrichloroethane/experimental study; Insulin/resistance/exposure to dichlorodiphenyltrichloroethane; Gene expression.
Resumo: | ABSTRACT Contact with the pesticide dichlorodiphenyltrichloroethane (p,p′-DDT) can be the cause of various harmful effects in humans, wildlife, and the environment. This pesticide is known to be persistent, lipophilic, resistant to degradation, and bioaccumulive in the environment and to be slowly released into bloodstream. Growing evidence shows that exposure to DDT is linked to type 2 diabetes mellitus. Individuals exposed to elevated levels of DDT and its metabolite have an increased prevalence of diabetes and insulin resistance. To evaluate these possible relationships, experiments were performed on eight-week-old female mice, divided into three groups (n = 10 per group): Group 1 received a vehicle-control intraperitoneal (i.p.) injection of sesame oil; Groups 2 and 3 received an i.p. dose of 50 and 100 µg/g p,p′-DDT respectively, dissolved in sesame oil. All groups were treated once daily for four days. Real-time PCR analysis of several genes was undertaken. Additionally, biochemical parameters and histopathological changes were measured. NQO1, HMOX1, NR1I3 and NR3C1 were up-regulated in DDT-exposed animals compared to the vehicle control group, while only SREBP1 was down-regulated in the 100 µg/g group. MTTP and FABP5, not previously reported for DDT exposure, but involved in regulation of fatty acid fluxes, could also function as biomarkers cross-talking between these signaling pathways. These results suggest that beyond epidemiological data, there is increasing molecular evidence that DDT may mimic different processes involved in diabetes and insulin resistance pathways.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200287
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502016000200007
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.52 n.2 2016
Fonte: | info:eu-repo/semantics/openAccess