Data/hora: 01/06/2024 19:05:31

Provedor de dados: 	61	
País: 	Brazil	
Título: 	Response to chitin in suspension-cultured Citrus aurantium cells	
Autores: 	Gallão,Maria I.; Cortelazzo,Angelo L.; Fevereiro,Manoel P. S.; Brito,Edy S. de.	
Data: 	2007-03-01	
Ano: 	2007	
Palavras-chave: 	Chitin; Citrus aurantium; Elicitation; L-phenylalanine ammonia-lyase; Peroxidase.	
Resumo: 	Morphological changes and enzyme activity in suspension-cultured cells of Citrus aurantium were followed after chitin elicitation. Chitin oligomers presented a rapid effect with a maximum activity after 3 h followed by a gradual decrease to the original levels at 8 h of incubation. Cell walls presented a lignification process and the cytoplasmatic protein became less reactive to anionic stains. In the treated material a reduction in starch grain size, an increase in the number of autophagic vacuoles, deposition of secretion in the pericellular space and a defibrillation of the cell-wall polymers were observed. Chitin oligomers increased peroxidase and L-phenylalanine ammonia-lyase activities. Extracellular peroxidase activity increased from 0.20 U mL-1 after 1 h to 0.45 U mL-1 at 3 h followed by a gradual decrease up to 8 h. The peroxidase isoenzyme pattern revealed a predominance of basic isoenzymes in these cells.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100008	
Editor: 	Brazilian Journal of Plant Physiology	
Relação: 	10.1590/S1677-04202007000100008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Plant Physiology  v.19 n.1 2007	
Fonte: 	info:eu-repo/semantics/openAccess