Data/hora: 11/06/2024 13:55:20

Provedor de dados: | 61
País: | Brazil
Título: | Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species
Autores: | Dias,Leonardo L.C.; Balbuena,Tiago S.; Silveira,Vanildo; Santa-Catarina,Claudete; Schevchenko,Andrej; Floh,Eny I. S.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | 2-DE; Comparative proteomics; Embryo development; Storage protein; Lauraceae.
Resumo: | The aim of the present work was to characterize changes in the protein profile throughout seed development in O. catharinensis, a recalcitrant species, by two-dimensional gel electrophoresis. Protein extraction was undertaken by using a thiourea/urea buffer, followed by a precipitation step with 10% TCA. Comparative analysis during seed development showed that a large number of proteins were exclusively detected in each developmental stage. The cotyledonary stage, which represents the transition phase between embryogenesis and the beginning of metabolism related to maturation, presents the highest number of stage-specific spots. Protein identification, through MS/MS analysis, resulted in the identification of proteins mainly related to oxidative metabolism and storage synthesis. These findings contribute to a better understanding of protein metabolism during seed development in recalcitrant seeds, besides providing information on established markers that could be useful in defining and improving somatic embryogenesis protocols, besides monitoring the development of somatic embryos in this species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100003
Editor: | Brazilian Journal of Plant Physiology
Relação: | 10.1590/S1677-04202010000100003
Formato: | text/html
Fonte: | Brazilian Journal of Plant Physiology  v.22 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess