Data/hora: 09/06/2024 02:47:37

Provedor de dados: | 62
País: | Brazil
Título: | Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae)
Autores: | SALVO,Ligia Maria; MALUCELLI,Maria Ivette Carboni; RICHARTZ,Rosária Regina Tesoni B.; BACILA,Metry.
Data: | 2000-01-01
Ano: | 2000
Palavras-chave: | Liver cells; Cell culture; Cells; Pisces; Metynnis roosevelti.
Resumo: | In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000500004
Editor: | Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
Relação: | 10.1590/S1413-95962000000500004
Formato: | text/html
Fonte: | Brazilian Journal of Veterinary Research and Animal Science  v.37 n.5 2000
Fonte: | info:eu-repo/semantics/openAccess