Data/hora: 05/06/2024 13:56:40

Provedor de dados: | 62
País: | Brazil
Título: | Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle
Autores: | DAFFNER,José; ABALOS,Pedro; PINOCHET,Lautaro; SCORTTI,Mariela; URCELAY,Santiago.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Brucellosis; Immunodiffusion tests; Vaccination; Cattle.
Resumo: | Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000300002
Editor: | Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
Relação: | 10.1590/S1413-95961999000300002
Formato: | text/html
Fonte: | Brazilian Journal of Veterinary Research and Animal Science  v.36 n.3 1999
Fonte: | info:eu-repo/semantics/openAccess