Data/hora: 10/06/2024 03:57:10

Provedor de dados: | 62
País: | Brazil
Título: | Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid
Autores: | ALVAREZ,Rafael Herrera; CARVALHO,João Batista Pereira de; ROSA E SILVA,Alzira; PERONE,Cibele Nunes; RIBELA,Maria Tereza Carvalho Pinto; OLIVEIRA FILHO,Enoch Borges de.
Data: | 1998-01-01
Ano: | 1998
Palavras-chave: | Superovulation; Follicular fluid; Passive immunization; Embryos; Cattle.
Resumo: | Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2alpha analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the experimental period and plasma concentrations of FSH, LH and progesterone were determined by radioimmunoassay. All cows from the immunized group, and 3 out of 5 cows in the control group, had more than 2 CL. There was no significant difference (P&gt;0.05) in the ovulation rate between immunized and control groups (14.4 and 9.9, respectively). The number of recovered embryos was not significantly different (P&gt;0.05) between groups, although cows immunized against bFF had a greater number of transferable embryos (3.4 ± 1.0 versus 0.8 ± 0.4, P&lt;0.05). Plasma gonadotrophin concentrations were not correlated with either ovulation rate or number of recovered embryos. Plasma progesterone concentration was positively correlated (r = 0.88, P&lt;0.01) to ovulation rate. Results suggested that passive immunization of cows with anti-bFF before superovulation did not reduce the variability of ovarian response.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600007
Editor: | Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
Relação: | 10.1590/S1413-95961998000600007
Formato: | text/html
Fonte: | Brazilian Journal of Veterinary Research and Animal Science  v.35 n.6 1998
Fonte: | info:eu-repo/semantics/openAccess