Data/hora: 02/06/2024 07:25:04

Provedor de dados: | 63
País: | Brazil
Título: | Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
Autores: | Monteiro,Mariza; Appezzato-da-Glória,Beatriz; Valarini,Maria José; Oliveira,Carlos Alberto de; Vieira,Maria Lucia Carneiro.
Data: | 2003-12-01
Ano: | 2003
Palavras-chave: | Plant biotechnology; In vitro culture; Morphogenesis; Forage legume.
Resumo: | Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162003000400012
Formato: | text/html
Fonte: | Scientia Agricola  v.60 n.4 2003
Fonte: | info:eu-repo/semantics/openAccess