Data/hora: 10/06/2024 15:16:16

Provedor de dados: 	63	
País: 	Brazil	
Título: 	Histochemical aspects of wheat resistance to leaf blast mediated by silicon	
Autores: 	Silva,Washington Luís da; Cruz,Maria Fernanda Antunes; Fortunato,Alessandro Antônio; Rodrigues,Fabrício Ávila.	
Data: 	2015-08-01	
Ano: 	2015	
Palavras-chave: 	Pyricularia oryzae; Flavonoids; Histochemistry; Phenolics.	
Resumo: 	Blast, caused by Pyricularia oryzae, has become a significant disease threat to wheat (Triticum aestivum L.) in Brazil. This study aimed to investigate at the histochemical level if silicon (Si) could enhance the production of flavonoids in the leaves of wheat plants in response to P. oryzae infection. Plants from the Aliança cultivar, which are susceptible to blast, were grown in hydroponic cultures containing 0 (-Si) or 2 mM of Si (+Si) and inoculated by spraying a conidial suspension of P. oryzae (1 × 105 conidia mL−1) on all adaxial leaf surfaces of plants at 60 days after emergence (growth stage 65). The fourth and fifth leaves of each plant were used to evaluate blast severity at 24, 36, 48, 72 and 96 h after inoculation (hai). At 96 hai, leaves were collected from plants to determine the foliar Si concentration. For cytological observations, leaf samples were randomly collected from the fourth and fifth leaves of each plant at 72 hai. The foliar Si concentration was higher in +Si plants (36 g kg−1) in comparison to -Si plants (2.6 g kg−1). This increased Si concentration was correlated with reduced fungal growth inside the epidermal cells and the development of blast symptoms on leaves. Strong fluorescence, which is an indication of the presence of flavonoids, was detected in the leaf cells of +Si plants using Neu’s and Wilson's reagents. A novel item of evidence is that, at the histochemical level, Si is involved in the potentiation of the biosynthetic pathway of flavonoids that increases wheat resistance to blast.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000400322	
Editor: 	São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"	
Relação: 	10.1590/0103-9016-2014-0221	
Formato: 	text/html	
Fonte: 	Scientia Agricola  v.72 n.4 2015	
Fonte: 	info:eu-repo/semantics/openAccess