Data/hora: 02/06/2024 15:30:27

Provedor de dados: | 63
País: | Brazil
Título: | Symbiotic efficiency and genetic characteristics of Bradyrhizobium sp. strain UFSM LA 1.3 isolated from Lupinus albescens (H. et Arn)
Autores: | Stroschein,Marcos Roberto Dobler; Eltz,Flávio Luiz Foletto; Antoniolli,Zaida Inês; Lupatini,Manoeli; Vargas,Luciano Kaiser; Giongo,Adriana; Pontelli,Mateus Padoin.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Lupin; Rhizobia; Genotypic approaches.
Resumo: | Legume species belonging to the genus Lupinus are annual herb plants. The majority of them are indigenous to the Americas. They are known for nitrogen-fixing symbioses with soil bacteria collectively called rhizobia. The aim of this study was to characterize a rhizobium strain isolated from Lupinus albescens using phenotypic, symbiotic and molecular approaches. Strain UFSM LA 1.3 was tested in vitro according to several parameters: colony size, color and growing rate; acid or alkaline reaction in yeast mannitol media supplemented with bromothymol blue; gum production. Molecular characterization was evaluated by PCR technique using primers BOX A1-R and sequence analysis of the 16S-23S rDNA intergenic region (ITS). ITS sequencing fragments showed genetic similarity with Bradyrhizobium sp. The polymorphism observed by BOX-PCR have shown that strain differs from the reference strain SEMIA 928 and SEMIA 938. The symbiotic efficiency under axenic conditions of UFSM LA 1.3 was 94.6%, without statistical differences compared to the mineral nitrogen fertilized control, to which was applied solution of 400 mg of ammonium nitrate.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600012
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162010000600012
Formato: | text/html
Fonte: | Scientia Agricola  v.67 n.6 2010
Fonte: | info:eu-repo/semantics/openAccess