Data/hora: 06/06/2024 11:56:59

Provedor de dados: | 63
País: | Brazil
Título: | Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a
Autores: | Melo,Flávia Mandolesi Pereira de; Fiore,Marli Fátima; Moraes,Luiz Alberto Beraldo de; Silva-Stenico,Maria Estela; Scramin,Shirlei; Teixeira,Manoel de Araújo; Melo,Itamar Soares de.
Data: | 2009-10-01
Ano: | 2009
Palavras-chave: | ESI-MS/MS; Pumilacidin; Endophytic bacteria; Bioautography.
Resumo: | In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162009000500002
Formato: | text/html
Fonte: | Scientia Agricola  v.66 n.5 2009
Fonte: | info:eu-repo/semantics/openAccess