Data/hora: 10/06/2024 11:19:42

Provedor de dados: 	63	
País: 	Brazil	
Título: 	Transient gene expression in electroporated intact tissues of Stylosanthes guianensis (Aubl.) Sw.	
Autores: 	Quecini,Vera Maria; Vieira,Maria Lúcia Carneiro.	
Data: 	2001-12-01	
Ano: 	2001	
Palavras-chave: 	GUS expression; Direct DNA transfer; Plant transformation; Forage legume.	
Resumo: 	Genetic transformation though protoplast electroporation has been established for commercially important plant species. In this work, explant sources, electric field strengths, electroporation buffers, DNA forms and osmotic pretreatment were assayed in order to optimize transient reporter gene expression in electroporated tissues of Stylosanthes guianensis, a tropical forage legume. Higher transformation rates were obtained employing cotyledonary explants and an electric field strength of 250 V cm-1. Linear plasmid DNA, chloridefree electroporation buffer and osmotic pretreatment with 1.6 mol L-1 mannitol also improved transient transformation but non-significantly. Transgene specific PCR amplification was employed to prove the transformed status of the tissues.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400018	
Editor: 	São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"	
Relação: 	10.1590/S0103-90162001000400018	
Formato: 	text/html	
Fonte: 	Scientia Agricola  v.58 n.4 2001	
Fonte: 	info:eu-repo/semantics/openAccess