Data/hora: 05/06/2024 07:38:55

Provedor de dados: 	64	
País: 	Brazil	
Título: 	Protoplast production from napier grass and pearl millet triploid hybrids	
Autores: 	Timbó,Ana Luiza de Oliveira; Davide,Lisete Chamma; Pinto,José Eduardo Brasil Pereira; Pereira,Antônio Vander.	
Data: 	2010-10-01	
Ano: 	2010	
Palavras-chave: 	Pennisetum purpureum; Pennisetum glaucum; In vitro cultivation; Interspecific hybrids; Forage.	
Resumo: 	The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020	
Editor: 	Editora da Universidade Federal de Lavras	
Relação: 	10.1590/S1413-70542010000500020	
Formato: 	text/html	
Fonte: 	Ciência e Agrotecnologia  v.34 n.5 2010	
Fonte: 	info:eu-repo/semantics/openAccess