Data/hora: 03/06/2024 11:08:24

Provedor de dados: | 65
País: | Brazil
Título: | Heparin-binding proteins of seminal plasma in Nellore bulls
Autores: | Fernandes,Carlos Eurico; Souza,Fabiana Ferreira de; Souza-Neto,Jayme Augusto; Ribola,Paulo Eduardo Martins.
Data: | 2009-02-01
Ano: | 2009
Palavras-chave: | Bovine; Proteins; Seminal plasma; Bos taurus indicus.
Resumo: | The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782008005000044
Formato: | text/html
Fonte: | Ciência Rural  v.39 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess