Data/hora: 05/06/2024 10:02:18

Provedor de dados: | 65
País: | Brazil
Título: | Molecular demonstration of intermittent shedding of Leptospira in cattle and sheep and its implications on control
Autores: | Rocha,Bruno Ribeiro; Narduche,Lorena; Oliveira,Clara Slade; Martins,Gabriel; Lilenbaum,Walter.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Cows; Intermittent; PCR; Sheep; Strain.
Resumo: | ABSTRACT: For a long time, it has been stated that urine leptospiral shedding is intermittent, which was observed primarily by culturing. However, culturing presents serious limitations, mainly low sensitivity, and failure on detection of leptospires cannot be neglected. PCR presents several advantages, mainly higher sensitivity. The present study aimed to analyze the occurrence of intermittency on leptospiral shedding by PCR in naturally and experimentally infected animals. In this study two experiments were conducted, the first with 60 cows naturally infected from an endemic herd. The second one was conducted in three sheep experimentally infected, each one with a different strain of Leptospira (strains Copenhageni L1-130, Canicola LO-4 and Pomona Fromm). Considering cattle, 43.3% presented negative in all tests, the remaining (56.7%) were positive at least once. From these, only one (1.6%) was positive in all samples, and seven (11.8%) were positive only in the last sampling, making it impossible to evaluate the intermittency. Noteworthy, 26 cows (43.3%) presented the typical intermittent pattern of leptospiral shedding in urine. In sheep, all experimentally infected animals presented the typical intermittent shedding patterns, independently of the inoculated leptospiral strain. We considered that a careful serial analysis of urine samples for a more definitive and reliable individual diagnosis would be required for a successful antimicrobial therapy and control of leptospirosis on a herd.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800453
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170088
Formato: | text/html
Fonte: | Ciência Rural  v.47 n.8 2017
Fonte: | info:eu-repo/semantics/openAccess