Data/hora: 09/06/2024 02:54:11

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil	
Autores: 	Flores,Mariana Martins; Mazaro,Renata Dalcol; Langohr,Ingeborg Maria; Roy,Alma; Strother,Keith; Fighera,Rafael Almeida.	
Data: 	2016-10-01	
Ano: 	2016	
Palavras-chave: 	Immunohistochemistry; Ki-67; Mutations; PCR; Mast cell tumor.	
Resumo: 	ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2% (27/39) were identified as low-grade and 30.8% (12/39) as high-grade by a 2-tier grading system. All tumors had a KIT expression pattern II, and 30.6% (11/36) had a high growth fraction (Ki-67). PCR amplification was successful in four of the 11 tumors examined. Two of these (50%) were positive for ITD. This study highlights the importance of using auxiliary techniques in the CCMT evaluation, identifies limitations and confirms the applicability of these methods on a routine diagnostic basis in Brazil. Our results will help to improve the prognostication of CCMTs in Brazilian diagnostic laboratories, encouraging the use of supplementary methods.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001804	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20160255	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.46 n.10 2016	
Fonte: 	info:eu-repo/semantics/openAccess