Data/hora: 01/06/2024 05:56:36

Provedor de dados: | 65
País: | Brazil
Título: | Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
Autores: | Telles,Thalita da Costa; Botelho,Ana Flávia Machado; Miranda,Ana Luisa Soares de; Soto-Blanco,Benito; Serakides,Rogéria; Ocarino,Natália de Melo; Melo,Marília Martins.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Viperidae envenomation; Stem cells; Rats; Myonecrosis; Muscular repair.
Resumo: | ABSTRACT: The aim of this study was to evaluate the use of mesenchymal stem cells (MSC) in the treatment of myonecrosis induced by Bothrops alternatus venom in rats. Seventy-five male adult Wistar rats were divided into three experimental groups. G1 and G2 were injected in the gastrocnemius muscle with 120μg of B. alternatus venom, while G3 received 200μL of PBS only. Three days after the venom injection, 12 rats from G1 were treated with 5.0 x 106 MSC in PBS, whereas G2 and G3 rats received PBS. Every three days, blood and muscle samples of five animals from each group were taken for serum biochemical and pathological analyses. Histological examinations showed more intense muscle lesions following MSC treatment, characterized by disorganization and loss of muscle fibers, with focal necrosis and inflammatory infiltration by mononuclear cells. In conclusion, the use of MSC for the treatment of local damage caused by inoculation of B. alternatus venom impaired muscle regeneration and interfered in the healing process.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300602
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170760
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.3 2018
Fonte: | info:eu-repo/semantics/openAccess