Data/hora: 06/06/2024 05:42:09

Provedor de dados: | 65
País: | Brazil
Título: | Correlation between chronic inflammation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum
Autores: | Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
Data: | 2016-11-01
Ano: | 2016
Palavras-chave: | Canine visceral leishmaniasis; Genital pathology; Immunohistochemistry; QPCR.
Resumo: | ABSTRACT: The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with  Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries.  L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102029
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20160137
Formato: | text/html
Fonte: | Ciência Rural  v.46 n.11 2016
Fonte: | info:eu-repo/semantics/openAccess