Data/hora: 05/06/2024 10:08:10

Provedor de dados: | 65
País: | Brazil
Título: | Qualitative and quantitative analysis of bacteria from vaginitis associated with intravaginal implants in ewes following estrus synchronization
Autores: | Vasconcelos,Carlos Otávio de Paula; Brandão,Felipe Zandonadi; Martins,Gabriel; Penna,Bruno; Souza-Fabjan,Joanna Maria Gonçalves de; Lilenbaum,Walter.
Data: | 2016-04-01
Ano: | 2016
Palavras-chave: | Intravaginal device; Microorganism; Sheep; Vaginal infection.
Resumo: | ABSTRACT: This study evaluated the presence of vaginitis and the bacterial load associated with different intravaginal implants in ewes. Twenty-four Dorper and crossbred ewes were allocated into three groups and received intravaginal implant containing 0.3g progesterone (CIDR(r)), 60mg MAP or sponges without progesterone (CONTROL) for six days. Then, CIDR and MAP treated-ewes received 12.5mg dinoprost and 300IU eCG. Vaginal mucus samples were collected at four times: before device insertion, at the day of its removal, 24 and 48 hours after. The samples were cultured and the colonies were counted (CFU/mL) and identified. The results obtained from the counting of CFU mL-1 were submitted to Kruskal-Wallis H test, with P&lt;0.05 being considered significant. Before device insertion, 68.2% of the samples yielded Staphylococcus spp. and 60.0% of them were Staphylococcus aureus. After implant removal, 100% of ewes had clinical signs of vaginitis. However, the level of local infection in the CONTROL-ewes was lower (P&gt;0.05) in comparison with MAP and CIDR-treated ewes. During the occurrence of vaginitis, the predominant isolates belonged to the coliform group, mainly Escherichia coli (72.7%). Such infection was not determined by the members of the vaginal microbiota that were present before implant insertion and normal microbiota was restored between 24 to 48 hours after insert removal.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400632
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20150365
Formato: | text/html
Fonte: | Ciência Rural  v.46 n.4 2016
Fonte: | info:eu-repo/semantics/openAccess