Data/hora: 01/06/2024 00:59:31

Provedor de dados: | 65
País: | Brazil
Título: | Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
Autores: | Okura,Mõnica Hitomi; Marin,José Moacir.
Data: | 2014-01-01
Ano: | 2014
Palavras-chave: | Escherichia coli; Cheese; STEC; ExPEC; Antimicrobial resistance; Minas frescal.
Resumo: | The soft cheese Minas frescal is one of the most popular cheese in Brazil, which is typically manufactured in small dairy farms under unsatisfactory hygiene conditions. To assess the risk involved in consumption of this cheese, virulence markers were investigated in 330 Escherichia coli strains isolated from 30 Minas frescal cheeses inspected by official government agency (SIF - serviço de inspeção federal), from 50 cheeses not inspected by SIF and 31 cheeses not inspected by SIF with spice added, all of them collected in the southwest of Minas Gerais State. The E. coli isolates were screened for the presence of Shiga toxin-encoding (stx 1 and stx 2), intimin (eae) genes and for the presence of (pap, sfa, afa) genes related to adhesion in epithelial cells. The only gene detected by PCR was the sfa gene at one isolate. The strains were also screened for resistance to 9 antimicrobial drugs. Predominant resistance was to cephalothin, tetracycline and streptomycin. Multidrug resistance was found among isolates from cheese with SIF (16.6%), cheese without SIF (8.0%) and cheese without SIF with spice added (30.0%) what is a reason for concern due to the high consumption of raw milk cheese by the Brazilian population.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801506
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20131237
Formato: | text/html
Fonte: | Ciência Rural  v.44 n.8 2014
Fonte: | info:eu-repo/semantics/openAccess