Data/hora: 12/06/2024 01:03:52

Provedor de dados: | 65
País: | Brazil
Título: | Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of  Solanum commersonii ssp.
Autores: | Tomé,Lívia Gracielle Oliveira; Silva,Adriano Bortolotti da; Pinto,Cesar Augusto Brasil Pereira; Davide,Lisete Chamma; Pereira,Dalvana Sousa; Carvalho,Carlos Roberto de.
Data: | 2016-11-01
Ano: | 2016
Palavras-chave: | Potato; Crop breeding; Flow citometry; Foliar anatomy.
Resumo: | ABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf anatomy was assessed by anatomic cuts. An increase was observed in the polar and equatorial diameter of stomata ("gigas effect") of the Solanum commersonii subsp. commersonii (SCC) and  Solanum commersonii subsp. malmeanum (SCM) clones, which was due to the use of the anti-mitotic substances. Treatments with colchicine (6.5mM; 72h) and oryzalin (50µM; 24h) caused death of the SCC plants cultured in vitro. In the others treatments of the SCC clone, the use of oryzalin and colchicine caused production of chimeric plants. The treatment of nodal segments of SCM with oryzalin (10-50µM; 24h) was effective on induction of tetraploid plants, which can be employed in genetic breeding programs in crossbreeding with cultured potato.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20150477
Formato: | text/html
Fonte: | Ciência Rural  v.46 n.11 2016
Fonte: | info:eu-repo/semantics/openAccess