Data/hora: 01/06/2024 02:30:09

Provedor de dados: | 65
País: | Brazil
Título: | Callus induction and plant regeneration of tomato (Lycopersicon esculentum cv. IPA 5) via anther culture
Autores: | Brasileiro,Ana Christina Rabello; Willadino,Lilia; Carvalheira,Gianna Griz; Guerra,Marcelo.
Data: | 1999-12-01
Ano: | 1999
Palavras-chave: | Growth regulators; Organogenesis; Tetraploid cells.
Resumo: | Different growth regulators combinations were tested on the production of anther callus in tomato cultivar IPA 5. Calli were induced on media supplemented with 1.0mgL-1 gibberellic acid (GA3), 0.05mgL-1 alpha-naphthaleneacetic acid (NAA) plus 0.1mgL-1 6-benzylaminopurine (BAP), or with 1.0mgL-1 BAP plus 1.0mgL-1 NAA. The medium containing 1.0mgL-1 BAP and 1.0mgL-1 NAA produced the highest calli frequency, and promoted plant regeneration by indirect organogenesis, when calli were transferred to 0.01mgL-1 BAP and 0.001mgL-1 NAA. Plants regenerated presented tetraploid cells and rare diploid cells. These tetraploid plants could be used as source for further obtainment of trisomic lines, for the purpose of genic localization studies and protein compounds analysis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000400008
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84781999000400008
Formato: | text/html
Fonte: | Ciência Rural  v.29 n.4 1999
Fonte: | info:eu-repo/semantics/openAccess