Data/hora: 11/06/2024 19:05:09

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Presence of multiple acid phosphatases activity in seedlings of cucumber, radish and rocket salad	
Autores: 	Tabaldi,Luciane Almeri; Ruppenthal,Raquel; Pereira,Luciane Belmonte; Cargnelutti,Denise; Gonçalves,Jamile Fabbrin; Morsch,Vera Maria; Schetinger,Maria Rosa Chitolina.	
Data: 	2008-06-01	
Ano: 	2008	
Palavras-chave: 	Cucumis sativus; Raphanus sativus; Eruca vesicaria; Phosphatases.	
Resumo: 	Acid phosphatases (3.1.3.2) are a group of enzymes widely distributed in nature, which catalyze the hydrolysis of a variety of phosphate esters in the pH range of 4-6. We confirmed the presence of acid phosphatases in seedlings of cucumber (Cucumis sativus), radish (Raphanus sativus) and rocket salad (Eruca vesicaria) under different assay conditions using a rapid and simple preparation. The results showed that the optimum pH and temperature used for all species were close to 5.5 and 35°C, respectively. The enzyme was inhibited by molybdate, fluoride, azide, levamisole, orthovanadate, Zn2+ and Cu2+. Suramin had no effect on enzyme activity. The acid phosphatase from cucumber, radish and rocket salad hydrolyzed a wide variety of phosphate esters and the highest activity was observed with PPi, ATP and GTP. These results demonstrate that the enzyme investigated in this study is different from well known ester phosphate cleaving plant enzymes (apyrase and inorganic pyrophosphatases) and this preparation could be a useful tool to future toxicological studies and to study initially all isoforms of acid phosphatase.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300009	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/S0103-84782008000300009	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.38 n.3 2008	
Fonte: 	info:eu-repo/semantics/openAccess