Data/hora: 02/06/2024 14:49:05

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes	
Autores: 	Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Triticum aestivum; Tissue culture; Growth regulators.	
Resumo: 	ABSTRACT:  The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant using the Hu and the Wu protocol, respectively. Adding 1mgL-1 2,4-D in the callus induction medium was the most favourable, producing 3.73 and 3.07 shoots per explant for PF020037 and BR18-Terena, respectively. In conclusion, a protocol for regeneration for two Brazilian wheat genotypes recommended and developed to be cultivated at the Cerrado region has been adapted.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20170806	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.48 n.11 2018	
Fonte: 	info:eu-repo/semantics/openAccess