Data/hora: 05/06/2024 11:39:59

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Staining fungal structures with artificial dyes used in the industry of juices	
Autores: 	Santana,Jeniffer Keterly Gonçalves; Seixas,André Leal; Ribeiro,Lucas Henrique Gonçalves; Cardoso,Ana Clara Santos; Rocha,Fernando da Silva; Fernandes,Maria de Fátima Gonçalves; Muniz,Maria de Fátima Silva.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Sunset yellow; Red bordeaux; Azo dyes; Fungal staining.	
Resumo: 	ABSTRACT: The objective of this research was to evaluate the efficiency of artificial dyes, sunset yellow and red bordeaux S, and the use of glycerol in different concentrations to consistently stain fungal structures in slides containing spores of Oidium sp., Albugo ipomoeae-panduratae, Pochonia chlamydosporia and hyphae of Phytopythium helicoides. Commercial product mixtures of the artificial dyes at 0.5, 1.0, 1.5, 2.0, 3.0 and 5.0% (w/v) added with glycerol at 0.25, 0.5 and 1.0% were evaluated. To stain chlamydospores, the suspension was placed in the staining solution or heated at 80ºC for 5 minutes. The slides were prepared by the wet mount slide method. Fungal spores were consistently stained starting at a concentration of 2% of the staining solution. The addition of glycerol to the staining solution improved the contrast of the sporangia, hyphae and chlamydospores. Higher intensity and uniformity of chlamydospore’s staining was verified using 3% dye solution and 1% heated glycerol, when compared to the unheated and blue-cotton solution.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900750	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20180071	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.48 n.9 2018	
Fonte: 	info:eu-repo/semantics/openAccess