Data/hora: 01/06/2024 04:31:06

Provedor de dados: | 65
País: | Brazil
Título: | Genotyping of South American clinical isolates of Pythium insidiosum based on single nucleotide polymorphism-based multiplex PCR
Autores: | Weiblen,Carla; Azevedo,Maria Isabel de; Ianiski,Lara Baccarin; Stibbe,Paula Cristina; Pereira,Daniela Isabel Brayer; Zanette,Régis Adriel; Sangioni,Luís Antônio; Rivero,Rodolfo; Santurio,Janio Morais; Botton,Sônia de Avila.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Pythium insidiosum; Pythiosis; Molecular detection; Genotype; Single nucleotide polimorphisms.
Resumo: | ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II, and the Thai isolate to clade III. P. aphanidermatum, P.torulosum, P.rhizo-oryzae and P.pachycaule voucher isolates were not amplified. For the first time, a P. insidiosum isolate from Uruguay, South America, was included in molecular analyzes. By SNP-based multiplex-PCR, it was possible to perform the identification and genotyping of the South American isolates of P. insidiosum, demonstrating similar genetic characteristics of these isolates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180744
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.1 2019
Fonte: | info:eu-repo/semantics/openAccess