Data/hora: 05/06/2024 10:35:44

Provedor de dados: | 65
País: | Brazil
Título: | Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants
Autores: | Batista,Diego Silva; Dias,Leonardo Lucas Carnevalli; Rêgo,Mailson Monteiro do; Saldanha,Cleber Witt; Otoni,Wagner Campos.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Micropropagation; Gas exchange; Ornamental plant; Capsicum annuum.
Resumo: | ABSTRACT: The influence of flask sealing and explant source on the in vitro morphogenesis of pepper, were evaluated in Capsicum annuum. Seeds were sterilized and inoculated in Murashige and Skoog media supplemented with vitamins, myo-inositol, sucrose, and agar. Gas exchange was evaluated in the germination stage by comparing 3 flask-sealing systems: rigid polypropylene lids (PLs) without vents, PLs with 1 vent, and PLs with 2 vents covered with membranes. In the regeneration stage, cotyledon and hypocotyl segments were transferred to the organogenesis-inducing media, being the different sealing types also tested in a factorial scheme. Photosynthetic pigments, morphological and hystological analyses were conducted for each treatment. Plants maintained in glass flasks capped with vented lids showed more vigorous growth and differentiated anatomical structures. These treatments resulted in taller plants, higher numbers and more expanded leaves, higher fresh and dry weights, and an increase in photosynthetic pigments. Cultivation of C. annuum in flasks with reduced gas exchange was more effective on callus induction. During the regeneration stage, hypocotyls were more effective than cotyledons. Sealing type influenced the morphogenic responses of pepper, demonstrating that an increase in gas exchange has a positive effect on biomass production and acclimatization of the plantlets.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300402
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20150245
Formato: | text/html
Fonte: | Ciência Rural  v.47 n.3 2017
Fonte: | info:eu-repo/semantics/openAccess