Data/hora: 14/06/2024 10:13:39

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland	
Autores: 	Costa,João Carlos Miguel; Espeschit,Isis de Freitas; Pieri,Fábio Alessandro; Benjamin,Laércio Anjos; Moreira,Maria Aparecida Scatamburlo.	
Data: 	2014-04-01	
Ano: 	2014	
Palavras-chave: 	Mammary gland; Escherichia coli; Milk; Acidity; Sugar.	
Resumo: 	Bacterial biofilms are involved in the aggravation and recurrence of clinical mastitis in dairy herds. Several factors such as pH, temperature, concentration of O2 and glucose can affect their induction and growth rates. In this study, biofilm production was demonstrated by 27 Escherichia coli strains isolated from bovine mastitis at different pH values depending on the availability of glucose, mimicking conditions found in mammary glands affected by the disease. Biofilm formation was analyzed by spectrophotometric analysis in microtiter plate with 16 different culture media and by scanning electron microscopy. Biofilm formation was greater in isolates cultured under conditions associated with low glucose availability (0.5% or 1.5%) and with either an acidic (5.5) or alkaline (8.5) pH, compared to conditions associated with high glucose availability (2.5% or 3.5%) and near-neutral pH (6.5 or 7.5). Results indicate possible favoring of biofilm production in the later stages of the infectious process caused by E. coli, when the gland environment is less propitious to bacterial growth due to the stress conditions mentioned above; contrasting with the environment of the healthy mammary gland, in which there is no limitation on nutrients or conditions of particular alkalinity or acidity. Thus, knowledge of the stage in which is the infection and environmental conditions of the mammary gland that cause increased production of biofilms is of paramount importance to guide the most appropriate control strategies to prevent relapse after treatment of bovine mastitis, an economically important disease in dairy cattle worldwide.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400015	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/S0103-84782014000400015	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.44 n.4 2014	
Fonte: 	info:eu-repo/semantics/openAccess