Data/hora: 17/06/2024 19:48:33

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Feline herpesvirus 1 viral load related to environmental factors in sheltered cats	
Autores: 	Baumworcel,Natasha; Pereira,Joylson de Jesus; Soares,Ana Maria Barros; Souza,Guilherme Nunes; Almosny,Nadia Regina Pereira; Castro,Tatiana Xavier de.	
Data: 	2019-01-01	
Ano: 	2019	
Palavras-chave: 	FHV-1; Conjunctivitis; Shelter medicine; QPCR; Animal shelter.	
Resumo: 	ABSTRACT:  Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20190067	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.49 n.5 2019	
Fonte: 	info:eu-repo/semantics/openAccess