Data/hora: 01/06/2024 04:33:28

Provedor de dados: | 65
País: | Brazil
Título: | Inducers and autoinducers on Salmonella enterica serovar Typhimurium motility, growth and gene expression
Autores: | Conceição,Rita de Cássia dos Santos da; Sturbelle,Régis Tuchtenhagen; Timm,Cláudio Dias; Leite,Fábio Pereira Leivas.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Quorum sensing; Salmonella; Epinephrine; Autoinducer; Motility.
Resumo: | Genus Salmonella bacteria are among the major pathogenic microorganisms in food. This bacterium pathogenicity is related to a number of virulence factors, among which its flagella. Flagellum expression is one of the virulence factors modulated by Quorum Sensing. Epinephrine produced by mammals uses the same signaling pathway of the 3 bacteria autoinducer. This study evaluated the effect of molecules inducer (epinephrine) and autoinducers (autoinducer 2 and autoinducer 3) and their association with the motility, growth and expression genes flhC, fliA, fliY, motA, motB e fliC of Salmonella Typhimurium (ST). Initially, ST was inoculated in BHI. Then, motility assays, growth curves and gene expression were performed by testing different concentrations of epinephrine (50, 125, 250, 500µM), conditioned medium (10 and 50%) and a combination of these. ST was exposed to different concentrations of epinephrine, conditioned medium and an association of both. Following, motility assays, bacterial growth and gene expression were performed. The results obtained showed that the combination of 500uM epinephrine with 50% conditioned medium increased ST bacterial motility by increasing the expression of genes involved in flagellum assembly.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202201
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20150295
Formato: | text/html
Fonte: | Ciência Rural  v.45 n.12 2015
Fonte: | info:eu-repo/semantics/openAccess